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Limited Proteolysis Coupled with Mass Spectrometry (LiP-MS)
Description
This template outlines the limited proteolysis coupled with mass spectrometry, or LiP-MS, workflow starting from retinal pigmented epithelial cells lysed and split into ELV34:6 treated and non-treated samples. The resulting protein mixtures undergo broad spectrum proteolysis, peptide fragmentation, and digestion before liquid chromatography, mass spectrometry, and machine learning based scoring generate a dose response plot of target peptides against ELV34:6 concentration.
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