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MALDI workflow
Description
This template outlines a MALDI-TOF mass spectrometry workflow for microbial identification, from preserved and fresh samples grown on 15 different media to second-generation colonies, protein extraction, and spotting protein extract with matrix onto a MALDI target plate. It continues through mass spectra generation on a Bruker Microflex system, dereplication and clustering with the SPeDE tool, and downstream analysis using BioNumerics dendrograms and identification report tables listing organism matches and scores.
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