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BioRender Template
Membrane Protein Extraction
Description
This template shows a stepwise protocol for extracting membrane proteins from a cell suspension, starting with centrifugation to form a cell pellet, then permeabilization and a second centrifugation to separate cytosolic proteins in the supernatant from membrane proteins in the pellet. The pellet is then resuspended in solubilization buffer and centrifuged again to collect purified membrane proteins in the final supernatant.
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