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Metabolic Assays - Using Seahorse Analyzers
Description
This template shows Seahorse analyzer output for a mitochondrial stress test measuring oxygen consumption rate after oligomycin, FCCP, and rotenone/antimycin A injections, and a glycolytic stress test measuring extracellular acidification rate after glucose, oligomycin, and 2-DG injections. Labeled regions on each graph indicate basal respiration, ATP-linked respiration, spare respiratory capacity, glycolysis, and glycolytic capacity.
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