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BioRender Template
Origin and Application of iPSCs
Creator
s
Description
For C9orf72 Hexanucleotide repeat expansion
iPSCs to MNs requires 3 kits: NIM → NEM → MND → MNM
NIM = Human ES/iPS Neural Induction Medium = Neural Rosettes
NEM = ENStem™-A Neural Expansion Medium = Neural Progenitor
MND = STEMdiff™ Motor Neuron Differentiation Kit = Motor Neurons
MNM = STEMdiff™ Motor Neuron Maturation Kit = terminally differentiated MNs
To ensure that we formed MNs = qPCR of markers linked to neuronal differentiation: SMI32 (NF-H), TUBB3 (TUJ), ISL1, NKX6.1, S100β and Nestin
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