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BioRender Template
MTT Assay
Description
This template outlines the five steps of an MTT cytotoxicity assay, from seeding cells into a well plate and adding MTT reagent, through a 3 to 4 hour incubation, to the colorimetric reaction and absorbance reading. It shows the chemical structures of MTT being converted into purple formazan crystals by mitochondrial reductase, and a plate reader measuring the resulting absorbance across the wells.
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