BioRender Template

Neutral Red Uptake Assay

Created by

Ellen Pires

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Description:

The neutral red uptake assay quantitatively estimates the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of viable cells to incorporate and bind the supravital dye neutral red in the lysosomes.

Updated

August 2026

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