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BioRender Template
Ni-NTA His-Tag Protein Purification
Creator
s
Description
This protocol outlines purification of His-tagged proteins using Ni-NTA affinity chromatography. His-tagged proteins bind selectively to nickel ions on Ni-NTA resin beads for purification from complex mixtures. Steps include resin preparation, protein binding, washing to remove contaminants, and elution using a competitive ligand. Quality assessment via SDS-PAGE ensures purity for downstream applications.
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