NMR Spectroscopy Workflow for Protein Structural Determination
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Description:
This template shows a four-stage NMR spectroscopy workflow for determining protein structure, beginning with preparing a 13C, 15N-labeled protein sample and acquiring NMR data on a spectrometer. It continues through processing a two-dimensional 1H-15N HSQC spectrum and assigning peaks, ending with structure calculation and refinement into an NMR ensemble of the protein's helical fold.
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This template shows a four-stage NMR spectroscopy workflow for determining protein structure, beginning with preparing a 13C, 15N-labeled protein sample and acquiring NMR data on a spectrometer. It continues through processing a two-dimensional 1H-15N HSQC spectrum and assigning peaks, ending with structure calculation and refinement into an NMR ensemble of the protein's helical fold.