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Paraoxonase (PON) lactonase activity assay
Creator
s
Description
Paraoxonases (PONs) can be measured indirectly by evaluating their enzymatic activities towards lactone substrates. The conversion of lactones into their corresponding acid forms can be determined by ultra high-performance liquid chromatography (UHPLC) coupled with mass spectrometry. They can be differentiated by their retention times, MS and MS/MS spectra.
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