Phage Plaque Assay
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Description:
This template shows a five-step phage plaque assay, from mixing overnight E. coli culture into soft agar and pouring it onto a TSA agar plate to adding phage suspension onto the double-layer agar. It finishes with overnight incubation at 36C and identification of the resulting phage plaques as clear spots on the bacterial lawn.
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This template shows a five-step phage plaque assay, from mixing overnight E. coli culture into soft agar and pouring it onto a TSA agar plate to adding phage suspension onto the double-layer agar. It finishes with overnight incubation at 36C and identification of the resulting phage plaques as clear spots on the bacterial lawn.