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BioRender Template
Preparation of competent cells (DHα-5)
Creator
s
Description
The figure outlines a protocol procedure for preparing competent cells, specifically DHα-5, integral in molecular biology, notably in vaccine research. the process begins with culturing DHα-5 in LB media in a shaker incubator for optimal growth. The resulting culture is aliquoted and sub-cultured in LB media to enhance cell density. Then, the culture undergoes centrifugation yielding a pellet. The pellet is then subjected to incubation on Ice. Following this CaCl2 is added and incubated on Ice
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