BioRender Template

Protoplast Polyethylene Glycol (PEG)-based Transfection

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Description: This template outlines a four-step protocol for PEG-based transfection of plant protoplasts, starting with purifying DNA and adding it with PEG to isolated protoplasts under gentle mixing. It continues by pelleting and washing the protoplasts twice to remove PEG, then storing or plating the transfected protoplasts on tissue culture plates with complete media.

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BioRender Template FAQs

What does the Protoplast Polyethylene Glycol (PEG)-based Transfection template show?

This template outlines a four-step protocol for PEG-based transfection of plant protoplasts, starting with purifying DNA and adding it with PEG to isolated protoplasts under gentle mixing. It continues by pelleting and washing the protoplasts twice to remove PEG, then storing or plating the transfected protoplasts on tissue culture plates with complete media.

Is the Protoplast Polyethylene Glycol (PEG)-based Transfection template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

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Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Protoplast Polyethylene Glycol (PEG)-based Transfection used for / who typically uses this template?

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