PTMScan Workflow
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Description:
This template shows the PTMScan workflow in three steps: protein preparation from cell culture through lysis, digestion, and solid phase extraction to isolate phosphopeptides; PTMScan analysis using a PTM-specific antibody bound to a bead for immunoprecipitation and peptide elution; and LC-MS/MS analysis followed by sequence assignment and relative quantitation of the peptides.
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This template shows the PTMScan workflow in three steps: protein preparation from cell culture through lysis, digestion, and solid phase extraction to isolate phosphopeptides; PTMScan analysis using a PTM-specific antibody bound to a bead for immunoprecipitation and peptide elution; and LC-MS/MS analysis followed by sequence assignment and relative quantitation of the peptides.








