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BioRender Template
Recombinant Monoclonal Antibody Production
Creator
s
Description
This figure outlines the workflow for recombinant monoclonal IgG antibody production. The process begins with cotransfection of plasmids encoding light chain (LC) and heavy chain (Fd, Fc) into host cells, followed by cell expansion. Productive clones are identified via stable cell screening, expanded, and preserved as cell stocks. Scaled-up cultures undergo Protein G purification, which isolates IgG antibodies. Quality control measures include Coomassie blue staining to verify the heavy and light chain integrity and IgG-specific ELISA to assess functionality.
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