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BioRender Template
Recombinant protein - cloning, expression and purification in bacteria
Description
This template outlines a ten-step protocol for cloning, expressing, and purifying a recombinant protein in E. coli, starting with cDNA amplification, cloning into a plasmid, and bacterial transformation. It continues through protein expression, bacterial lysis, a solubility assay separating soluble and insoluble fractions, SDS-PAGE and western blot analysis, purification, dialysis, and functional activity assays such as platelet aggregation and MTT.
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