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BioRender Template
Sequence and Ligation Independent Cloning
Creator
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Description
Sequence and Ligation Independent Cloning (SLIC) is a standardized cloning method that relies on in vitro homologous recombination. This template summarizes the method introduced by Li and Elledge. DNA regions with homology to a target vector are added to a target gene by PCR, and 5′ overhangs are generated by one of three methods. The vector and insert are annealed, yielding an incomplete recombination intermediate, which is transformed into cells and repaired.
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