BioRender Template

Sequence and Ligation Independent Cloning

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Blugene Plasmidmaker

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Description: Sequence and Ligation Independent Cloning (SLIC) is a standardized cloning method that relies on in vitro homologous recombination. This template summarizes the method introduced by Li and Elledge. DNA regions with homology to a target vector are added to a target gene by PCR, and 5′ overhangs are generated by one of three methods. The vector and insert are annealed, yielding an incomplete recombination intermediate, which is transformed into cells and repaired.

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BioRender Template FAQs

What does the Sequence and Ligation Independent Cloning template show?

Sequence and Ligation Independent Cloning (SLIC) is a standardized cloning method that relies on in vitro homologous recombination. This template summarizes the method introduced by Li and Elledge. DNA regions with homology to a target vector are added to a target gene by PCR, and 5′ overhangs are generated by one of three methods. The vector and insert are annealed, yielding an incomplete recombination intermediate, which is transformed into cells and repaired.

Is the Sequence and Ligation Independent Cloning template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Sequence and Ligation Independent Cloning used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.