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Sequencing Technique GeoMx™ Spatial Analysis of RNA in FFPE Tissue Samples

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Jamerson Silva

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Description: This describes GeoMx™ RNA assay for multiplexed mRNA detection in FFPE tissues. It uses ISH probes with barcodes, processed in GeoMx DSP Instrument for RNA profiling in specific regions. FFPE sections are prepped, incubated with probes, washed, and labeled for morphology. Regions of interest are profiled, and indices quantified using NanoString nCounter® for digital RNA expression with spatial context.

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This describes GeoMx™ RNA assay for multiplexed mRNA detection in FFPE tissues. It uses ISH probes with barcodes, processed in GeoMx DSP Instrument for RNA profiling in specific regions. FFPE sections are prepped, incubated with probes, washed, and labeled for morphology. Regions of interest are profiled, and indices quantified using NanoString nCounter® for digital RNA expression with spatial context.

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