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BioRender Template
Sheep and goatpox virus detection using CRISPR Cas12a combined with lateral flow strips
Creator
s
Description
the genomic DNA extracted from the clinical isolates sheep and goatpox virus and the extracted DNA amplified using a recombinase polymerase amplification (RPA) at 39 degree Celsius for 20 minutes . then the RPA products is added into the CRISPR Cas12a reaction and incubate for for 20 minutes at 37 degree Celsius. finally the RPA-Cas12a reaction is combined with lateral flow strips to read the results.
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