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Shotgun Sequencing Sanger vs NGS
Description
This template compares shotgun sequencing by the Sanger method and next generation sequencing (NGS), both starting from template DNA fragmented into pieces. The Sanger method proceeds through cloning, bacterial transformation, and automated sequencing cycles to produce a chromatogram, while the NGS method uses adapter ligation, a library array, and digital imaging to produce overlapping sequence reads that are reconstructed into contigs.
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