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BioRender Template
Staining and Imaging of Tetrahymena for DNA Damage Markers
Description
This template shows a ten-step protocol for staining and imaging Tetrahymena cells to detect DNA damage markers, beginning with primary and secondary cultures and proceeding through PFA fixation, Triton permeabilization, and blocking with bovine serum albumin. It continues with primary antibody staining against Rad51 or gamma-H2A.X, secondary antibody and DAPI labeling, slide mounting, and fluorescence microscopy imaging.
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