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BioRender Template
STED microscopy
Description
This template compares conventional confocal imaging to STED microscopy, showing how overlapping the excitation laser with a donut-shaped depletion laser narrows the effective excitation spot and produces a smaller point spread function for fluorescence emission. A second panel shows a Jablonski-style energy diagram explaining how stimulated emission depletion suppresses fluorescence at the donut edges to increase resolution.
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