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BioRender Template
Steps of a high-throughput CRISPR screen
Creator
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Description
High-throughput CRISPR screen: Synthesize guide RNA library, clone into plasmids, create lentivirus library. Transduce into cell pools, screen for phenotype. Positive selection enriches surviving cells with targeted gRNAs; negative selection identifies deceased cells with depleted gRNAs. Next-generation-sequencing and bioinformatics compare gRNA abundance, revealing gene loci linked to phenotypes.
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