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subcellular fractionation of mouse primary astrocyte

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Julia Bal

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Description: This template outlines a subcellular fractionation protocol for mouse primary astrocytes starting from a DIV47 culture with microglia shaken off. It shows sequential centrifugation steps using nuclei lysis and wash buffers to separate the cytoplasmic extract, nuclei, nucleoplasmic extract, and a chromatin-enriched pellet.

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What does the subcellular fractionation of mouse primary astrocyte template show?

This template outlines a subcellular fractionation protocol for mouse primary astrocytes starting from a DIV47 culture with microglia shaken off. It shows sequential centrifugation steps using nuclei lysis and wash buffers to separate the cytoplasmic extract, nuclei, nucleoplasmic extract, and a chromatin-enriched pellet.

Is the subcellular fractionation of mouse primary astrocyte template free to use?

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