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BioRender Template
T cell activation assay
Description
This template outlines a six step T cell activation assay, starting with PBMC preparation and T cell isolation, followed by culturing T cells with activation media in a 96 well plate. Later steps include a 72 hour incubation, treatment with Golgi stop, PMA, and ionomycin, antibody staining for CD4, CD8, perforin, granzyme B, and IFN-gamma, and final analysis by flow cytometry.
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