BioRender Template

T7E1 assay for the detection of indels after CRISPR-based gene editing

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Dario Palmieri

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Description: This template shows the T7E1 assay workflow, starting with DNA extraction and PCR amplification of cells, followed by denaturation and reannealing of wild-type and indel-containing strands to form homoduplexes and heteroduplexes. A gel schematic at the bottom shows how T7E1 treatment cleaves heteroduplexes, with the homoduplex to heteroduplex band ratio indicating the abundance of CRISPR-induced indels in the cell population.

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What does the T7E1 assay for the detection of indels after CRISPR-based gene editing template show?

This template shows the T7E1 assay workflow, starting with DNA extraction and PCR amplification of cells, followed by denaturation and reannealing of wild-type and indel-containing strands to form homoduplexes and heteroduplexes. A gel schematic at the bottom shows how T7E1 treatment cleaves heteroduplexes, with the homoduplex to heteroduplex band ratio indicating the abundance of CRISPR-induced indels in the cell population.

Is the T7E1 assay for the detection of indels after CRISPR-based gene editing template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

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