Thin- film hydration method for preparation of Niosomes(MLV's)

In the thin-film hydration (TFH) method, all lipids are dissolved in an organic solvent such as chloroform. The solvent is then evaporated under reduced pressure, and the hydration mixture containing water-soluble components is added at a temperature above the phase transition temperature of the surfactants used. TFH is the most common technique for forming niosomes, often producing multilamellar vesicles (MLV) with a wider particle size distribution without a size reduction process.

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