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Three-Dimensional Superresolution Fluorescence Microscopy of the Nuclear Pore Complex
Description
This template outlines a workflow for 3D superresolution fluorescence microscopy of the nuclear pore complex, starting with fluorophore labeling of nucleoporins like RANBP2, NUP107, NUP133, ELYS, SEH1, and TPR followed by two-color 3D single molecule localization imaging and channel alignment and averaging. The resulting top, side, and tilted views map the ring-shaped scaffold structure, cytoplasmic filaments, and nuclear basket components of the nuclear pore complex.
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