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BioRender Template
Transformation using λ Red recombination
Description
This schematic outlines the transformation into E. coli BW25113/pIJ790 which involves PCR amplification, electroporation, antibiotic selection and sequencing verification. The process integrates oriT and integrase cassette into the S. coelicolor cosmid (pUHA3) using λ Red recombination (pIJ790).
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