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Trypanosoma cruzi infection colorimetric assay

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Tamara Sternlieb

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Description: An essay to screen treatment effects on T. cruzi amastigote intracellular growth in mammalian cells. For this assay parasites expressing the bacterial B-galactosidase (lacZ) (ATCC PRA-330) are treated and then inoculated on wells containing a monolayer of mammalian cells. The infection proceedes for 48 h and then cell lysis and addition of the galactosidase substrate CPRG (chlorophenol red‐β‐D‐galactopyranoside) allows for parasite load measurement by reading absorbance between 570 and 595 nm.

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An essay to screen treatment effects on T. cruzi amastigote intracellular growth in mammalian cells. For this assay parasites expressing the bacterial B-galactosidase (lacZ) (ATCC PRA-330) are treated and then inoculated on wells containing a monolayer of mammalian cells. The infection proceedes for 48 h and then cell lysis and addition of the galactosidase substrate CPRG (chlorophenol red‐β‐D‐galactopyranoside) allows for parasite load measurement by reading absorbance between 570 and 595 nm.

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